GlutaredoxinV1, Main, Exploration, bibRecord, 001349

Identification and reactivity of the catalytic site of pig liver thioltransferase.

Identifieur interne : 001349 ( Main/Exploration ); précédent : 001348; suivant : 001350

Identification and reactivity of the catalytic site of pig liver thioltransferase.

Auteurs : Z R Gan ; W W Wells

Source :

The Journal of biological chemistry [ 0021-9258 ] ; 1987.

RBID : pubmed:3571279

Descripteurs français

KwdFr :
- Acide iodo-acétique (MeSH), Animaux (MeSH), Cinétique (MeSH), Cystine (métabolisme), Foie (enzymologie), Glutarédoxines (MeSH), Iodo-acétates (métabolisme), Oxidoreductases (métabolisme), Protein-disulfide reductase (glutathione) (MeSH), Radio-isotopes du carbone (MeSH), Sites de fixation (MeSH), Suidae (MeSH).
MESH :
- enzymologie : Foie.
- métabolisme : Cystine, Iodo-acétates, Oxidoreductases.
- Acide iodo-acétique, Animaux, Cinétique, Glutarédoxines, Protein-disulfide reductase (glutathione), Radio-isotopes du carbone, Sites de fixation, Suidae.

English descriptors

KwdEn :
- Animals (MeSH), Binding Sites (MeSH), Carbon Radioisotopes (MeSH), Cystine (metabolism), Glutaredoxins (MeSH), Iodoacetates (metabolism), Iodoacetic Acid (MeSH), Kinetics (MeSH), Liver (enzymology), Oxidoreductases (metabolism), Protein Disulfide Reductase (Glutathione) (MeSH), Swine (MeSH).
MESH :
- chemical , metabolism : Cystine, Iodoacetates, Oxidoreductases.
- chemical : Carbon Radioisotopes, Glutaredoxins, Iodoacetic Acid, Protein Disulfide Reductase (Glutathione).
- enzymology : Liver.
- Animals, Binding Sites, Kinetics, Swine.

Abstract

The active site cysteine of pig liver thioltransferase was identified as Cys22. The kinetics of the reaction between Cys22 of the reduced enzyme and iodoacetic acid as a function of pH revealed that the active site sulfhydryl group had a pKa of 2.5. Incubation of reduced enzyme with [1-14C]cysteine prevented the inactivation of the enzyme by iodoacetic acid at pH 6.5, and no stable protein-cysteine disulfide was found when the enzyme was separated from excess [1-14C]cysteine, suggesting an intramolecular disulfide formation. The results suggested a reaction mechanism for thioltransferase. The thiolated Cys22 first initiates a nucleophilic attack on a disulfide substrate, resulting in the formation of an unstable mixed disulfide between Cys22 and the substrate. Subsequently, the sulfhydryl group at Cys25 is deprotonated as a result of micro-environmental changes within the active site domain, releasing the mixed disulfide and forming an intramolecular disulfide bond. Reduced glutathione, the second substrate, reduces the intramolecular disulfide forming a transient mixed disulfide which is then further reduced by glutathione to regenerate the reduced enzyme and form oxidized glutathione. The rate-limiting step for a typical reaction between a disulfide and reduced glutathione is proposed to be the reduction of the intramolecular disulfide form of the enzyme by reduced glutathione.

PubMed: 3571279

Affiliations:

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Le document en format XML

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<author><name sortKey="Gan, Z R" sort="Gan, Z R" uniqKey="Gan Z" first="Z R" last="Gan">Z R Gan</name>
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<author><name sortKey="Wells, W W" sort="Wells, W W" uniqKey="Wells W" first="W W" last="Wells">W W Wells</name>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals (MeSH)</term>
<term>Binding Sites (MeSH)</term>
<term>Carbon Radioisotopes (MeSH)</term>
<term>Cystine (metabolism)</term>
<term>Glutaredoxins (MeSH)</term>
<term>Iodoacetates (metabolism)</term>
<term>Iodoacetic Acid (MeSH)</term>
<term>Kinetics (MeSH)</term>
<term>Liver (enzymology)</term>
<term>Oxidoreductases (metabolism)</term>
<term>Protein Disulfide Reductase (Glutathione) (MeSH)</term>
<term>Swine (MeSH)</term>
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<keywords scheme="KwdFr" xml:lang="fr"><term>Acide iodo-acétique (MeSH)</term>
<term>Animaux (MeSH)</term>
<term>Cinétique (MeSH)</term>
<term>Cystine (métabolisme)</term>
<term>Foie (enzymologie)</term>
<term>Glutarédoxines (MeSH)</term>
<term>Iodo-acétates (métabolisme)</term>
<term>Oxidoreductases (métabolisme)</term>
<term>Protein-disulfide reductase (glutathione) (MeSH)</term>
<term>Radio-isotopes du carbone (MeSH)</term>
<term>Sites de fixation (MeSH)</term>
<term>Suidae (MeSH)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Cystine</term>
<term>Iodoacetates</term>
<term>Oxidoreductases</term>
</keywords>
<keywords scheme="MESH" type="chemical" xml:lang="en"><term>Carbon Radioisotopes</term>
<term>Glutaredoxins</term>
<term>Iodoacetic Acid</term>
<term>Protein Disulfide Reductase (Glutathione)</term>
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<term>Oxidoreductases</term>
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<term>Binding Sites</term>
<term>Kinetics</term>
<term>Swine</term>
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<keywords scheme="MESH" xml:lang="fr"><term>Acide iodo-acétique</term>
<term>Animaux</term>
<term>Cinétique</term>
<term>Glutarédoxines</term>
<term>Protein-disulfide reductase (glutathione)</term>
<term>Radio-isotopes du carbone</term>
<term>Sites de fixation</term>
<term>Suidae</term>
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<front><div type="abstract" xml:lang="en">The active site cysteine of pig liver thioltransferase was identified as Cys22. The kinetics of the reaction between Cys22 of the reduced enzyme and iodoacetic acid as a function of pH revealed that the active site sulfhydryl group had a pKa of 2.5. Incubation of reduced enzyme with [1-14C]cysteine prevented the inactivation of the enzyme by iodoacetic acid at pH 6.5, and no stable protein-cysteine disulfide was found when the enzyme was separated from excess [1-14C]cysteine, suggesting an intramolecular disulfide formation. The results suggested a reaction mechanism for thioltransferase. The thiolated Cys22 first initiates a nucleophilic attack on a disulfide substrate, resulting in the formation of an unstable mixed disulfide between Cys22 and the substrate. Subsequently, the sulfhydryl group at Cys25 is deprotonated as a result of micro-environmental changes within the active site domain, releasing the mixed disulfide and forming an intramolecular disulfide bond. Reduced glutathione, the second substrate, reduces the intramolecular disulfide forming a transient mixed disulfide which is then further reduced by glutathione to regenerate the reduced enzyme and form oxidized glutathione. The rate-limiting step for a typical reaction between a disulfide and reduced glutathione is proposed to be the reduction of the intramolecular disulfide form of the enzyme by reduced glutathione.</div>
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<Month>06</Month>
<Day>19</Day>
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<DateRevised><Year>2013</Year>
<Month>11</Month>
<Day>21</Day>
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<Issue>14</Issue>
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<Day>15</Day>
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<Title>The Journal of biological chemistry</Title>
<ISOAbbreviation>J Biol Chem</ISOAbbreviation>
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<ArticleTitle>Identification and reactivity of the catalytic site of pig liver thioltransferase.</ArticleTitle>
<Pagination><MedlinePgn>6704-7</MedlinePgn>
</Pagination>
<Abstract><AbstractText>The active site cysteine of pig liver thioltransferase was identified as Cys22. The kinetics of the reaction between Cys22 of the reduced enzyme and iodoacetic acid as a function of pH revealed that the active site sulfhydryl group had a pKa of 2.5. Incubation of reduced enzyme with [1-14C]cysteine prevented the inactivation of the enzyme by iodoacetic acid at pH 6.5, and no stable protein-cysteine disulfide was found when the enzyme was separated from excess [1-14C]cysteine, suggesting an intramolecular disulfide formation. The results suggested a reaction mechanism for thioltransferase. The thiolated Cys22 first initiates a nucleophilic attack on a disulfide substrate, resulting in the formation of an unstable mixed disulfide between Cys22 and the substrate. Subsequently, the sulfhydryl group at Cys25 is deprotonated as a result of micro-environmental changes within the active site domain, releasing the mixed disulfide and forming an intramolecular disulfide bond. Reduced glutathione, the second substrate, reduces the intramolecular disulfide forming a transient mixed disulfide which is then further reduced by glutathione to regenerate the reduced enzyme and form oxidized glutathione. The rate-limiting step for a typical reaction between a disulfide and reduced glutathione is proposed to be the reduction of the intramolecular disulfide form of the enzyme by reduced glutathione.</AbstractText>
</Abstract>
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Identification and reactivity of the catalytic site of pig liver thioltransferase.

Identification and reactivity of the catalytic site of pig liver thioltransferase.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki